Review




Structured Review

Croda International Plc acyl coenzyme a
Acyl Coenzyme A, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acyl+coa/Coenzyme+A/pm41543688-53-0-5
Average 95 stars, based on 131 article reviews
acyl coenzyme a - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

In Vitro:

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.
Article Snippet: .. In vitro AGPAT2 activity assay Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 minutes at 37 °C. ..

Activity Assay:

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.
Article Snippet: .. In vitro AGPAT2 activity assay Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 minutes at 37 °C. ..

Article Title: Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer.
Article Snippet: .. Briefly, equal amounts of protein were combined with defatted bovine serum albumin (A6003; Sigma–Aldrich), N-[6-[(7-nitrobenzo-2oxa-1,3-diazol-4-yl)–sphinganine (810206P; Avanti Polar Lipids) and C16:0 (870716P) or C24:1 acyl CoA (870725P; Avanti Polar Lipids) and incubated at 37 C. C16:0 was used to assess CerS5/6 activities, whereas C24:1 assessed CerS2 activity. ..

Isolation:

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.
Article Snippet: .. In vitro AGPAT2 activity assay Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 minutes at 37 °C. ..

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis
Article Snippet: To test the effect of antibodies on ER tubules, freshly purified ER (7.5 μg of protein in 50 μl) was incubated with 12.5 μg/ml of TOM20 antibody (612278, BD Biosciences) or DRP1 antibody (611113, BD Biosciences) in a buffer containing 150 mM KCl and 20 mM MES (pH 7.0) at room temperature for 3 h. Electron microscopy sample preparation, negative staining, and length analysis were performed as described above. .. Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 min at 37 °C. ..

Incubation:

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.
Article Snippet: .. In vitro AGPAT2 activity assay Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 minutes at 37 °C. ..

Article Title: Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer.
Article Snippet: .. Briefly, equal amounts of protein were combined with defatted bovine serum albumin (A6003; Sigma–Aldrich), N-[6-[(7-nitrobenzo-2oxa-1,3-diazol-4-yl)–sphinganine (810206P; Avanti Polar Lipids) and C16:0 (870716P) or C24:1 acyl CoA (870725P; Avanti Polar Lipids) and incubated at 37 C. C16:0 was used to assess CerS5/6 activities, whereas C24:1 assessed CerS2 activity. ..

Article Title: AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis
Article Snippet: To test the effect of antibodies on ER tubules, freshly purified ER (7.5 μg of protein in 50 μl) was incubated with 12.5 μg/ml of TOM20 antibody (612278, BD Biosciences) or DRP1 antibody (611113, BD Biosciences) in a buffer containing 150 mM KCl and 20 mM MES (pH 7.0) at room temperature for 3 h. Electron microscopy sample preparation, negative staining, and length analysis were performed as described above. .. Freshly isolated ER (30 μg of proteins) was incubated with 50 μM 18:0 acyl-CoA (870718, Avanti) and 10 μM lysoPA-TopFluor (810280, Avanti) in 200 μl of 100 mM Tris-HCl (pH 7.4) containing 1 mg/ml of fatty acid-free bovine serum albumin (A7030, Sigma) for 10 min at 37 °C. ..

Sonication:

Article Title: Unravelling neuronal and glial differences in ceramide composition, synthesis, and sensitivity to toxicity
Article Snippet: Cells were lysed and scraped on ice using HEPES buffer (20 mM HEPES, 25 mM KCl, 250 mM Sucrose, 2 mM MgCl 2 ) made according to kit (640011, 640012, 640014, Avanti Polar Lipids Inc.) instructions . .. Lysates, NBD-sphinganine (810206 P, Avanti Polar Lipids Inc.), and Acyl-CoA (18:0, 870718 P, Avanti Polar Lipids Inc.; 16:0, 870716 P, Avanti Polar Lipids Inc.; 24:1, 870725 P, Avanti Polar Lipids Inc.) for reaction were water-bath sonicated prior to the reaction. ..

Article Title: Unravelling neuronal and glial differences in ceramide composition, synthesis, and sensitivity to toxicity
Article Snippet: Cells were lysed and scraped on ice using HEPES buffer (20 mM HEPES, 25 mM KCl, 250 mM Sucrose, 2 mM MgCl2) made according to kit (640011, 640012, 640014, Avanti Polar Lipids Inc.) instructions ( ). .. Lysates, NBD-sphinganine (810206P, Avanti Polar Lipids Inc.), and Acyl-CoA (18:0, 870718P, Avanti Polar Lipids Inc.; 16:0, 870716P, Avanti Polar Lipids Inc.; 24:1, 870725P, Avanti Polar Lipids Inc.) for reaction were water-bath sonicated prior to the reaction. ..



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